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The weekly SNL schedule was also grueling

Following incubation, the cells were washed and stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA), 750 ng/mL ionomycin, and 10 g/mL brefeldin A (all from eBioscience) for 4 h at 37 C in a 5% CO 2 environment